Do you use conventional PCR or QPCR?
QPCR has many advantages over conventional PCR and in our opinion is the method of choice for diagnostic PCR.
Do you run internal amplification controls in ALL your PCR tests?
Internal amplification controls are essential to confirm the presence of amplifiable DNA in the sample. They allow us to detect false negative results and hence provide a much more reliable diagnostic service.
Do you have separate laboratories for each step of the PCR process to reduce contamination risks?
Separation of PCR stages (extraction of DNA, setting up the PCR and running of the PCR) is an essential requirement for any laboratory undertaking PCR; failure to separate the different steps in the PCR process will lead to false positive results.
Are ALL your tests developed and validated by experienced molecular biologists?
PCR and QPCR are complex molecular reactions and require an in-depth understanding of molecular biology if sensitive, specific and reliable diagnostic tests are to be produced. The Molecular Diagnostic Unit within Langford Veterinary Services is run by Dr Chris Helps who is a leading QPCR molecular biologist. He was recently the recipient of the 2008 BSAVA Amoroso award for his major contribution to the development of PCR tests for the diagnosis of many feline infectious diseases. Dr Helps works alongside a number of clinical pathologists, clinicians and technicians with extensive molecular biology experience.
How long has your laboratory been running QPCR tests?
Developing and running QPCR diagnostic tests takes time if done properly. The technical staff must be highly trained and work to a very high standard. The longer the laboratory has been running QPCR tests the more experienced it will be. The Molecular Diagnostic Unit within Langford Veterinary Services has been exclusively using QPCR for the past 7 years, during which time over 24,000 samples have been processed.
Do you have peer reviewed publications reporting validation of the QPCR tests you offer?
QPCR tests offered should always be supported by extensive research and development of publishable quality in the veterinary scientific literature. This shows the laboratory’s commitment to producing high quality QPCR tests and validates their use in diagnostic work. Publications produced by staff in the Molecular Diagnostic Unit can be found on our website.