Molecular Microbiology Diagnostics

The molecular microbiology laboratory is a growing part of Langford Vets' portfolio and processes samples for bacterial and fungal identification from small and farm animals.

qPCR assay

Our highly skilled staff offer a fast turnaround time as there is no waiting for cultures to grow.  All of our qPCR assays are designed and validated by our highly experienced molecular biologists.  We also benefit from input from clinicians and our own researchers in the School of Veterinary Science, University of Bristol.  In conjunction with the clinicians and researchers, we are constantly developing new tests and tests for emerging diseases.

Fluorescent in situ hybridisation (FISH) assay

Liver or gut samples are assayed for the presence of bacteria and upon request any individual species.

Sample submission:  Please ensure samples are freshly fixed in formalin or sent as slides/blocks.

Fungal sensitivity testing

Fungi are tested for resistance to commonly used antifungal agents.

Please see below for a summary of our tests:

Fungal sensitivity testing

Culture identification and sensitivity testing for resistance to commonly used antifungal agents.  

Fluorescent in situ hybridisation (FISH) assay

Fluorescent probes are bound to the DNA of target bacteria present in the sectioned biopsy.  Liver or gut samples are assayed for the presence or absence of all bacteria and specific species upon request.

Sample submission:  Please ensure samples are freshly fixed in formalin or sent as slides/blocks. 

qPCR tests

Clostridia toxins qPCR **NEW**

E. coli qPCR **NEW**

Feline/Canine faecal bacteriology screen
A qPCR for Salmonella, Campylobacter jejuni, C. coli, C. upsaliensis and E.coli pathovars EPEC, VTEC (stx1 and 2), ETEC.

Campylobacter speciation (C. jejuni, C. coli and C. upsaliensis) from faeces
A qPCR test for the speciation of Campylobacter into pathogenic and non-pathogenic types.

Leptospira from blood samples
A qPCR for the presence of pathogenic Leptospira species.

Samples should be blood (preferred) or fresh urine.  Blood sent in EDTA.

Rapid screen for bacteria (CSF/joint fluid)
Quantitative screen for the presence of bacteria.

Sheep footrot screen
A qPCR screen for Dichelobacter, Treponema and Fusobacteria for the diagnosis of ovine footrot.

Sheep footrot serotyping
Serotyping for Dichelobacter from ovine footrot.