The protozoal parasite Tritrichomonas foetus (TF) is now well recognised as a cause of chronic diarrhoea in cats. It is a particular problem in multi-cat households, notably pedigree breeding catteries and rescue shelters, where one or more cats within the group are usually affected.
TF-associated diarrhoea is most often seen in cats under 1 year of age, but it has also been reported in older cats. The parasite targets the large bowel causing colitis, with frequent passage of small quantities of liquid to semi-formed faeces often with blood, mucus and straining. Some affected cats develop faecal incontinence.
Infection can also be diagnosed by culturing the organism using the commercially available InPouch™ TF kit, which has been marketed for the diagnosis of TF infection in cattle. However, the InPouch™ method is laborious and time consuming since pouch contents need to be examined daily by microscopy and results can only be considered negative after 12 days. Additionally the specificity of the InPouch™ system is unknown as a positive result does not preclude the possibility of infection with trichomonads other than TF.
More recently faecal PCR has been recommended as the diagnostic test of choice for TF infection, being more sensitive than both direct examination and culture by the InPouch™ method. However, PCR on faeces can be problematic due to the PCR-inhibitory effect of many substances that are co-purified with the DNA during extraction.
A real-time quantitative (Q)PCR has recently been developed by the Molecular Diagnostic Unit, Langford Veterinary Services for the detection and quantification of TF in faecal samples. This new multiplex assay is the first to use an internal amplification control PCR alongside the TF PCR, enabling detection of any inhibitory substances present in the extracted DNA, which could cause false negative TF results.
The use of QPCR in this new assay also allows us to report the relative amount of TF present in the faeces, which may be useful in monitoring the response to treatment.
A dosage of 30 mg/kg orally once daily for two weeks has been suggested. Infrequent cases of neurotoxicosis have been reported with drug administration and it is teratogenic so must be handled with care.
Consultation with a feline specialist is advised before embarking on treatment.
The diarrhoea will usually resolve spontaneously in untreated cats although this may take some time; months or more. Cats in which clinical signs (diarrhoea) have resolved seem to continue to excrete the organism for periods of up to two years.
Faecal samples for Tritrichomonas foetus QPCR are less stable than many other sample types: to ensure the best results possible, samples should ideally be received in the laboratory within 3 days of sampling, although positive results have been obtained after lengthier delays.
Referring vets who have diagnosed T.foetus by PCR are asked to contact Professor Danielle Gunn-Moore or Stephanie Lalor at University of Edinburgh Hospital for Small Animals using the contact details below to discuss enrolment of cats into the study.
Tel. 01316 507650